The research is focused on ribonucleotide reductase, a key enzyme in the synthesis of the DNA building blocks, dNTPs. One aim is to conditionally knock out the ribonucleotide reductase large subunit (R1) in differentiated mammalian cells to study:
i) The need for de novo dNTP synthesis for DNA repair in differentiated cells.
ii) The need for de novo dNTP synthesis for mitochondrial DNA replication and repair.
iii) The composition of the ribonucleotide reductase enzyme in differentiated cells. We also want to identify ribonucleotide reductase-interacting proteins using immunoprecipitations and chromatin immunopreciptations from synchronized mammalian cell extracts.

Methods:
The studies involve standard cell and molecular biology and biochemical techniques. Position description: The position is available for the initial 2 years as a tax-exempt fellowship with a possibility of extension. Location: The Department of Medical Biochemistry and Biophysics consists of 15 independent groups employing around 80 people. The department is located within the Chemical Biological Centre at Umeå University and provides well- equipped common facilities and excellent platforms in a creative, inspiring and highly interactive environment.
Fellowship Application Deadline: 31 January 2011
Further Fellowship Information and Application